FASL antibody

Catalog No. ABIN120320

The Mouse Monoclonal anti-FASL antibody has been validated for FACS, IP and EIA. It is suitable to detect FASL in samples from Human.

Quick Overview for FASL antibody (ABIN120320)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 14C2

Product Details anti-FASL Antibody

Specificity: This antibody recognises the CD178 cell surface antigen. This antibody may be used in combination with clone 10F2 (SM2268P) in ELISA assays. Clone 14C2 is not reported to have a blocking function. We recommend the use of SM2268LE for functional studies.

Purification: Affinity chromatography on Protein G

Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/25 - 1/50, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 / Fas Ligand

Background: CD178 is a 40 kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6

Gene ID: 356

NCBI Accession: NP_000630

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity